ons with poor research contribution. Policies regarding open access publications, international research collaboration, and adoption of e-learning methodologies in low- and middle-income countries need to be endorsed.
Data presented can be used to develop and enhance e-learning in health sciences education in regions with poor research contribution. Policies regarding open access publications, international research collaboration, and adoption of e-learning methodologies in low- and middle-income countries need to be endorsed.Academic medicine professionals spend their careers striving for promotion and standing in their respective institutions and the global scientific community. Publishing in high-impact journals aids in that pursuit; yet, formal coursework and training rarely emphasize scientific writing, making it difficult to gain the skills necessary to succeed. The authors implemented an intramural peer-review service in the medical school of a preeminent university to offer guidance, resources, and hands-on writing assistance at no cost. This program model bridges a gap in scientific writing instruction, boosts academic productivity, and increases opportunities to publish in higher impact journals.The online version contains supplementary material available at 10.1007/s40670-021-01251-9.
The online version contains supplementary material available at 10.1007/s40670-021-01251-9.The use of arts-based knowledge translation (KT) methods such as video remains underutilized in the research arena, despite the strong influence and reach of technology in society. This paper provides a detailed description of the process involved in producing a video as a novel KT strategy to respond to and address findings from our research on professional socialization experiences of physiotherapy students. Specifically, the video challenged dominant stereotypes regarding the profession by depicting the realities and rewards of modern physiotherapy practice. Using a guiding KT framework, this paper provides insight on how researchers can disseminate their study findings in an impactful way using multimedia. Creative outlets such as video and social media are innovative dissemination tools that enable KT to have a powerful and lasting impact.Structured illumination microscopy (SIM) is a widely used imaging technique that doubles the effective resolution of widefield microscopes. Most current implementations rely on diffractive elements, either gratings or programmable devices, to generate structured light patterns in the sample. These can be limited by spectral efficiency, speed, or both. Here we introduce the concept of fiber SIM that allows for camera frame rate limited pattern generation and manipulation over a broad wavelength range. Illumination patterns are generated by coupling laser beams into radially opposite pairs of fibers in a hexagonal single mode fiber array where the exit beams are relayed to the microscope objective's back focal plane. https://www.selleckchem.com/products/e-7386.html The phase stepping and rotation of the illumination patterns are controlled by fast electro-optic devices. We achieved a rate of 111 SIM frames per second and imaged with excitation patterns generated by both 488 nm and 532 nm lasers.Lasers with ultrashort pulse durations have become ubiquitous in various applications, including ocular surgery. Therefore, we need to consider the role of nonlinear optical effects, such as supercontinuum generation during propagation within the ocular media, when evaluating their potential hazard. We used a NIR femtosecond laser to generate a supercontinuum within an artificial eye. We recorded the visible spectra of the supercontinuum generated and calculated the energy contained within the visible band. Our results indicate that for wavelengths between 1350 nm and 1450 nm the energy contained within the visible band of the generated white light supercontinuum may surpass current safety exposure limits, and pose a risk of injury to the retina.This study demonstrates that chlorophosphonazo III (CPZ III) can be used as a contrast agent for photoacoustic calcium imaging. CPZ III can pass across the plasma membrane for labeling intracellular Ca2+ without cytotoxicity. In optical-resolution photoacoustic microscopy (OR-PAM), the photoacoustic (PA) signal intensity was strongly correlated with the presence of CPZ III and Ca2+ at various concentrations. The sensitivity of PA signal reception was enhanced by using an 8 MHz single-element focused ultrasound detector due to their matched frequency characteristics. Differences in the PA signal intensity were successfully found between the core and margin areas of tumorspheres in three-dimensional cell cultures. These findings indicate that CPZ III can serve as a novel PA contrast agent for functional Ca2+ imaging using OR-PAM.In this study, we use non-linear imaging microscopy to characterize the structural properties of porous collagen-GAG scaffolds (CGS) seeded with human umbilical vein endothelial cells (HUVECs), as well as human mesenchymal stem cells (hMSCs), a co-culture previously reported to form vessel-like structures inside CGS. The evolution of the resulting tissue construct was monitored over 10 days via simultaneous two- and three-photon excited fluorescence microscopy. Time-lapsed 2- and 3-photon excited fluorescence imaging was utilized to monitor the temporal evolution of the vascular-like structures up to 100 ?m inside the scaffold up to 10 days post-seeding. 3D polarization-dependent second harmonic generation (PSHG) was utilized to monitor collagen-based scaffold remodeling and determine collagen fibril orientation up to 200 ?m inside the scaffold. We demonstrate that polarization-dependent second harmonic generation can provide a novel way to quantify the reorganization of the collagen architecture in CGS simultaneously with key biomechanical interactions between seeded cells and CGS that regulate the formation of vessel-like structures inside 3D tissue constructs. A comparison between samples at different days in vitro revealed that gradually, the scaffolds developed an orthogonal net-like architecture, previously found in real skin.